597 papers
This study characterizes the diverse regulatory outcomes of introducing over 20 human cancer mutations into the Drosophila Notch Negative Regulatory Region, revealing distinct mechanisms of constitutive activation and synergy with PEST deletions that depend on the specific mutation location and its impact on protein stability and ligand responsiveness.
The paper introduces SteMClass, a novel DNA methylation-based classifier that accurately and harmoniously identifies various iPSC differentiation states across different protocols and array versions, thereby establishing a standardized quality control framework to enhance reproducibility and accelerate clinical translation in regenerative medicine.
This study reveals that early cytokinetic events in zebrafish dorsal forerunner cells instructively drive cytoskeletal reorganization, actin recruitment, and rosette formation, which are essential for the Kupffer vesicle's detachment from the enveloping layer and subsequent lumen morphogenesis.
This study identifies GEM, a metastable 34-nm nano-liquid hub on the plasma membrane that physically integrates growth and immune-evasion signaling through LLPS-like clustering, thereby amplifying survival pathways and promoting tumor growth in vivo.
This study identifies a novel mechanosensitive PIEZO1-ANGPT2-ITGA9 signaling loop in Schlemm's canal endothelium that regulates intraocular pressure, revealing that disruption of this pathway leads to glaucoma and highlighting it as a promising therapeutic target.
This study demonstrates that cardiac REDD1 promotes glucose oxidation and hypertrophic growth by suppressing PPARα activity and its downstream targets (such as PDK4) through an mTORC1-independent mechanism.
The paper introduces CytoType and its simplified variant ESM-CE, species-specific models that leverage pre-trained protein embeddings to achieve cell type classification performance comparable to large foundation models while using orders of magnitude fewer parameters and offering greater interpretability.
The paper introduces EpiFlow, a robust, high-throughput spectral flow cytometry platform capable of simultaneously quantifying 16 epigenetic markers at single-cell resolution across diverse species and biological contexts, enabling precise cell-type classification and drug effect analysis.
This study provides a comprehensive molecular characterization of ATDC5-derived cartilage organoids through time-resolved transcriptomic and proteomic analyses, revealing a detailed profile of chondrogenic differentiation and previously unreported extracellular matrix components to enhance the model's utility for studying human genetic skeletal diseases.
This study reveals that Greatwall kinase drives acute myeloid leukemia cell proliferation through a non-canonical mechanism involving the direct phosphorylation of MARK3 to regulate cytokinesis, rather than the previously established ENSA-PP2A-B55 pathway.
This study demonstrates that repeated administration of mesenchymal stromal cells (MSCs) during ex vivo lung perfusion and the early post-transplant period, rather than a single dose or the specific cell source, effectively restores function and prevents graft dysfunction in severely injured porcine donor lungs, offering a promising regenerative strategy to expand the organ pool for transplantation.
This study demonstrates that Huh7 cells cultured in physiologically relevant media exhibit metabolic responses and lipid accumulation patterns comparable to primary human hepatocytes, supporting their utility as a scalable in vitro model for studying metabolic dysfunction-associated steatotic liver disease (MASLD).
This study demonstrates that the histone acetyltransferase HAT1 is essential for maintaining intestinal stem cell homeostasis by regulating chromatin structure in lamina-associated domains to control stem cell proliferation and differentiation.
This study reveals that cardiomyocyte autophagy, regulated by AP-1 transcription factors, is essential for driving heart regeneration in adult zebrafish by facilitating cardiomyocyte protrusion into injured tissue and modulating macrophage phenotypes from pro-inflammatory to pro-angiogenic and pro-fibrotic states to ensure effective scar resolution.
This study identifies syndecan-1 as a critical driver of alveolar type 2 epithelial cell senescence and impaired lung repair in pulmonary fibrosis, demonstrating that its age- and disease-associated upregulation disrupts epithelial regeneration while its genetic loss attenuates senescence and preserves function.
This study introduces the integrated CLEAP-scMAPS pipeline, a novel single-cell multi-omics platform that enables unbiased transcriptome-proteome co-profiling of rare cerebrospinal fluid circulating tumor cells, revealing that malignant dormancy and post-transcriptional buffering are key mechanisms driving chemotherapeutic resistance in leptomeningeal metastasis.
During Fc gamma receptor-mediated phagocytosis, the ER-resident phosphatase PTP1B localizes to actin-cleared membrane contact sites to dephosphorylate Syk and suppress the Syk-Shc1-NOX2 signaling axis, thereby limiting excessive superoxide production.
This study demonstrates that sustained exposure to the inflammatory secretome of activated CAR-T cells directly impairs human hematopoietic stem cell function through IFNγ and TNFα signaling, causing prolonged cytopenias that can be reversed by dual cytokine blockade without compromising antitumor efficacy.
This study reveals that Aurora kinase A (AURKA) and the mitophagy receptor PHB2 form a platform of inner mitochondrial membrane interactors with respiratory chain proteins to spatially regulate ATP production and organelle architecture, thereby driving metabolic heterogeneity in cancer cells.
This study demonstrates that the pharmacological NRF2 activator Omaveloxolone upregulates NRF2-target proteins and modestly increases SMN protein levels in Spinal Muscular Atrophy type I fibroblasts, which exhibit diminished basal NRF2 pathway output compared to controls.